TY - JOUR
T1 - Overexpression of tetraspanin CO-029 in hepatocellular carcinoma
AU - Kanetaka, Kengo
AU - Sakamoto, Michiie
AU - Yamamoto, Yoshiya
AU - Yamasaki, Susumu
AU - Lanza, François
AU - Kanematsu, Takashi
AU - Hirohashi, Setsuo
N1 - Funding Information:
This work was supported by a Grant-in-Aid for the Research on Human Genome and Gene Therapy and a Grant-in-Aid for the Second Term Comprehensive 10-year Strategy for Cancer Control from the Ministry of Health and Welfare of Japan. K.K. and Y.Y. are the recipients of Research Resident Fellowships from the Foundation for Promotion of Cancer Research in Japan.
PY - 2001
Y1 - 2001
N2 - Background/Aims: The molecules involved in the progression of hepatocellular carcinoma (HCC) are not fully understood. The aim of this study was to elucidate the crucial genes involved in cancer progression and metastasis. Methods: Selectively expressed genes were screened using differential display analysis, and then further analyzed by real-time quantitative reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: Tetraspanin CO-029 was found to be frequently and significantly overexpressed in HCC. Real-time quantitative RT-PCR showed that the CO-029 mRNA level was 1.7 times higher (P = 0.030) in cancerous tissues than in non-cancerous tissues. mRNA expression of the other tetraspanins, CD9 and CD82, was downregulated in HCC, especially in tumors with intrahepatic spreading (portal vein invasion and/or intrahepatic metastasis). In contrast, mRNA expression of CO-029 tended to be increased in cancerous tissue showing intrahepatic spreading compared with tumors without such spreading. Immunohistochemical analysis revealed that CO-029 was overexpressed in poorly differentiated HCCs compared with well to moderately differentiated tumors (P < 0.001), and in HCCs showing intrahepatic spreading compared with those without spreading (P = 0.019). Conclusions: Our findings suggest that CO-029 has some roles in the promotion of metastasis of HCC.
AB - Background/Aims: The molecules involved in the progression of hepatocellular carcinoma (HCC) are not fully understood. The aim of this study was to elucidate the crucial genes involved in cancer progression and metastasis. Methods: Selectively expressed genes were screened using differential display analysis, and then further analyzed by real-time quantitative reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: Tetraspanin CO-029 was found to be frequently and significantly overexpressed in HCC. Real-time quantitative RT-PCR showed that the CO-029 mRNA level was 1.7 times higher (P = 0.030) in cancerous tissues than in non-cancerous tissues. mRNA expression of the other tetraspanins, CD9 and CD82, was downregulated in HCC, especially in tumors with intrahepatic spreading (portal vein invasion and/or intrahepatic metastasis). In contrast, mRNA expression of CO-029 tended to be increased in cancerous tissue showing intrahepatic spreading compared with tumors without such spreading. Immunohistochemical analysis revealed that CO-029 was overexpressed in poorly differentiated HCCs compared with well to moderately differentiated tumors (P < 0.001), and in HCCs showing intrahepatic spreading compared with those without spreading (P = 0.019). Conclusions: Our findings suggest that CO-029 has some roles in the promotion of metastasis of HCC.
KW - CO-029
KW - Differential display
KW - Disease progression
KW - Hepatocellular carcinoma
KW - Immunohistochemistry
KW - Metastasis
KW - Real-time quantitative reverse-transcription polymerase chain reaction
KW - Tetraspanin
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U2 - 10.1016/S0168-8278(01)00183-0
DO - 10.1016/S0168-8278(01)00183-0
M3 - Article
C2 - 11690710
AN - SCOPUS:0035166399
SN - 0168-8278
VL - 35
SP - 637
EP - 642
JO - Journal of Hepatology
JF - Journal of Hepatology
IS - 5
ER -
