TY - JOUR
T1 - Oxiradical-dependent photoemission induced by a phacoemulsification probe
AU - Shimmura, S.
AU - Tsubota, K.
AU - Oguchi, Y.
AU - Fukumura, D.
AU - Suematsu, M.
AU - Tsuchiya, M.
N1 - Funding Information:
Thisworkwas supported by the BrainResearch Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2016M3C7A1905477, NRF-2014M3C7A1046050) and the Basic Science Research Program through the NRF funded by the Ministry of Education (NRF-2017R1D1A1B03036423). This study was approved by the Institutional Review Board of Gwangju Institute of Science and Technology (IRB no. 20180629-HR-36-07-04). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.
Funding Information:
Acknowledgements This work was supported by the Brain Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2016M3C7A1905477, NRF-2014M3C7A1046050) and the Basic Science Research Program through the NRF funded by the Ministry of Education (NRF-2017R1D1A1B03036423). This study was approved by the Institutional Review Board of Gwangju Institute of Science and Technology (IRB no. 20180629-HR-36-07-04). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.
PY - 1992
Y1 - 1992
N2 - Oxygen free radical formation by conventional phacoemulsification devices has been postulated as a possible mechanism of corneal endothelial damage during surgery. To test this hypothesis, phacoemulsification probe-induced free radical production was visualized using a single photon-counting camera and an O2--sensitive luciferin derivative, 2-methyl-6-[p-methoxyphenyl-3,7- dihydroimidazo [1,2-a]pyrazin-3-one (MCLA), which allows the visualization of spatial and temporal alterations in free radical production. Within 1 min after starting ultrasound emission, MCLA-dependent chemiluminescence was increased significantly, the intensity of which was maximal at the tip of the probe and tapered along a gradient toward distal portions. The chemiluminescence was suppressed significantly by adding either superoxide dismutase (300 U/ml) or sodium azide (20 mmol/l). By adding deuterium to the medium, MCLA-dependent chemiluminescence significantly increased, suggesting the involvement of singlet oxygen in the reaction.
AB - Oxygen free radical formation by conventional phacoemulsification devices has been postulated as a possible mechanism of corneal endothelial damage during surgery. To test this hypothesis, phacoemulsification probe-induced free radical production was visualized using a single photon-counting camera and an O2--sensitive luciferin derivative, 2-methyl-6-[p-methoxyphenyl-3,7- dihydroimidazo [1,2-a]pyrazin-3-one (MCLA), which allows the visualization of spatial and temporal alterations in free radical production. Within 1 min after starting ultrasound emission, MCLA-dependent chemiluminescence was increased significantly, the intensity of which was maximal at the tip of the probe and tapered along a gradient toward distal portions. The chemiluminescence was suppressed significantly by adding either superoxide dismutase (300 U/ml) or sodium azide (20 mmol/l). By adding deuterium to the medium, MCLA-dependent chemiluminescence significantly increased, suggesting the involvement of singlet oxygen in the reaction.
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M3 - Article
C2 - 1326494
AN - SCOPUS:0026768753
SN - 0146-0404
VL - 33
SP - 2904
EP - 2907
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 10
ER -