Paper-Based Device for Naked Eye Urinary Albumin/Creatinine Ratio Evaluation

Ryuya Hiraoka, Kento Kuwahara, Yun Chiao Wen, Tzung Hai Yen, Yuki Hiruta, Chao Min Cheng, Daniel Citterio

研究成果: Article査読

42 被引用数 (Scopus)

抄録

This paper introduces the concept of "Drawing-PADs" (Drawing paper-based microfluidic analytical devices) allowing to intuitively evaluate the urinary albumin (Alb) index, a clinically important parameter used for the early detection of renal deficiencies related to diabetes, among others. To enable regular monitoring of the Alb index, a simple examination method suitable for self-diagnosis is highly desirable. The Drawing-PADs rely on the simultaneous naked eye detection of Alb and creatinine (Cre) on a single device according to the distance-based microfluidic PAD (μPAD) approach. The Alb index is visualized by simply drawing a straight line connecting the top of two color-changed assay channel sections (Alb and Cre channels), followed by visually confirming the position of the intercept of the drawn straight line. The semiquantitative Alb index evaluation performed with Drawing-PADs does not require any equipment such as a camera, software, or a color reference chart. The obtained results are independent of the sample volume and are not influenced by changes in the absolute Alb and Cre concentrations caused by urine excretion variations, making spot urine assays possible. Classification of Alb index values according to clinically relevant criteria (normoalbuminuria, microalbuminuria, and macroalbuminuria) is readily achieved within 15 min and has been validated for 15 human urine samples including diabetic patients and healthy volunteers.

本文言語English
ページ(範囲)1110-1118
ページ数9
ジャーナルACS Sensors
5
4
DOI
出版ステータスPublished - 2020 4月 24

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 器械工学
  • プロセス化学およびプロセス工学
  • 流体および伝熱

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