PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

Yuki Hiruta; Takaaki Funatsu; Minami Matsuura; Jian Wang; Eri Ayano; Hideko Kanazawa

doi:10.1016/j.snb.2014.10.065

PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

Yuki Hiruta, Takaaki Funatsu, Minami Matsuura, Jian Wang, Eri Ayano, Hideko Kanazawa

研究成果: Article › 査読

36 被引用数 (Scopus)

抄録

A pH/temperature-responsive fluorescence polymer, I₄₂D₅₀S₈-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I₄₂D₅₀S₈-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I₄₂D₅₀S₈-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.

本文言語	English
ページ（範囲）	724-731
ページ数	8
ジャーナル	Sensors and Actuators, B: Chemical
巻	207
号	PartA
DOI	https://doi.org/10.1016/j.snb.2014.10.065
出版ステータス	Published - 2015 2月

ASJC Scopus subject areas

電子材料、光学材料、および磁性材料
器械工学
凝縮系物理学
表面、皮膜および薄膜
金属および合金
電子工学および電気工学
材料化学

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title = "PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake",

abstract = "A pH/temperature-responsive fluorescence polymer, I42D50S8-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I42D50S8-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I42D50S8-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.",

keywords = "Acidic tumor microenvironment, Cellular uptake, Fluorescence polymer, pH",

author = "Yuki Hiruta and Takaaki Funatsu and Minami Matsuura and Jian Wang and Eri Ayano and Hideko Kanazawa",

note = "Funding Information: This work was supported in part by Grant-in-Aid for Research Activity Start-Up (Grant No. 25893232 ) from Japan Society for Promotion of Science (JSPS). ",

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doi = "10.1016/j.snb.2014.10.065",

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T1 - PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

AU - Hiruta, Yuki

AU - Funatsu, Takaaki

AU - Matsuura, Minami

AU - Wang, Jian

AU - Ayano, Eri

AU - Kanazawa, Hideko

N1 - Funding Information: This work was supported in part by Grant-in-Aid for Research Activity Start-Up (Grant No. 25893232 ) from Japan Society for Promotion of Science (JSPS).

PY - 2015/2

Y1 - 2015/2

N2 - A pH/temperature-responsive fluorescence polymer, I42D50S8-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I42D50S8-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I42D50S8-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.

AB - A pH/temperature-responsive fluorescence polymer, I42D50S8-FL, consisting of N-isopropylacrylamide, N,N-dimethylacrylamide, and sulfamethazineacrylamide was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, followed by activation of terminal carboxyl groups with N-hydroxysuccinimide and reaction with 5-aminofluorescein (FL). I42D50S8-FL exhibited a pH-dependent phase transition across the lower critical solution temperature (LCST), with specific intracellular uptake observed only in weak acidic conditions. I42D50S8-FL has the potential to be applied for the selective imaging of acidic tumor microenvironments.

KW - Acidic tumor microenvironment

KW - Cellular uptake

KW - Fluorescence polymer

KW - pH

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JO - Sensors and Actuators, B: Chemical

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PH/temperature-responsive fluorescence polymer probe with pH-controlled cellular uptake

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