Physiologically based pharmacokinetic model for pralmorelin hydrochloride in rats

Risa Nasu, Yoichi Kumagai, Hirokuni Kogetsu, Masayuki Tsujimoto, Hisakazu Ohtani, Yasufumi Sawada

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Pralmorelin hydrochloride (pralmorelin), consisting of six amino acid residues, is a growth hormone-releasing peptide. The aim of this study is to analyze the pharmacokinetics of pralmorelin after intravenous bolus administration to rats, and to develop a physiologically based pharmacokinetic (PB-PK) model to describe and predict the concentrations of pralmorelin in blood and tissues. Pralmorelin (3 mg/kg) was administered intravenously to 24 Sprague-Dawley rats. Groups of three rats were sacrificed by decapitation at each designated time point (up to 4 h), and plasma and tissues (brain, lung, heart, liver, kidney, small intestine, muscle, adipose, and skin) were collected. Bile was also pooled until decapitation. The concentration of pralmorelin in samples was determined by liquid chromatography-tandem mass spectrometry. Plasma concentrations of pralmorelin declined rapidly in a biexponential manner. Biliary excretion of pralmorelin was so rapid that 80% of the dose was recovered unchanged in the bile within 1 h after administration. The distribution parameters in each tissue were obtained by using a hybrid model and an integration plot. They revealed that the distribution of pralmorelin into liver was blood flow-limited, and its distribution was permeability-limited in all other tissues. The PB-PK model developed in this study well described the time courses of pralmorelin concentration in the blood and tissues of rats.

本文言語English
ページ(範囲)1488-1494
ページ数7
ジャーナルDrug Metabolism and Disposition
33
10
DOI
出版ステータスPublished - 2005 10月
外部発表はい

ASJC Scopus subject areas

  • 薬理学
  • 薬科学

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