Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes Centrosome Maturation

Yuko Ozaki, Hirotaka Matsui, Hiroya Asou, Akiko Nagamachi, Daisuke Aki, Hiroaki Honda, Shin'ichiro Yasunaga, Yoshihiro Takihara, Tadashi Yamamoto, Shunsuke Izumi, Miho Ohsugi, Toshiya Inaba

研究成果: Article査読

52 被引用数 (Scopus)


During prometaphase, dense microtubule nucleation sites at centrosomes form robust spindles that align chromosomes promptly. Failure of centrosome maturation leaves chromosomes scattered, as seen routinely in cancer cells, including myelodysplastic syndrome (MDS). We previously reported that the Miki (LOC253012) gene is frequently deleted in MDS patients, and that low levels of Miki are associated with abnormal mitosis. Here we demonstrate that Miki localizes to the Golgi apparatus and is poly(ADP-ribosyl)ated by tankyrase-1 during late G2 and prophase. PARsylated Miki then translocates to mitotic centrosomes and anchors CG-NAP, a large scaffold protein of the γ-tubulin ring complex. Due to impairment of microtubule aster formation, cells in which tankyrase-1, Miki, or CG-NAP expression is downregulated all show prometaphase disturbances, including scattered and lagging chromosomes. Our data suggest that PARsylation of Miki by tankyrase-1 is a key initial event promoting prometaphase.

ジャーナルMolecular Cell
出版ステータスPublished - 2012 9月 14

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学


「Poly-ADP Ribosylation of Miki by tankyrase-1 Promotes Centrosome Maturation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。