Preparation of conophylline affinity nano-beads and identification of a target protein

Eriko Suzuki, Hideki Ogura, Kuniki Kato, Izumi Takei, Yasuaki Kabe, Hiroshi Handa, Kazuo Umezawa

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Conophylline, a vinca alkaloid extracted from the tropical plant Ervatamia microphylla, has been shown to induce the differentiation of insulin-producing β-cells in cultured cells and in animals. However, its mechanism of action and the molecular target have remained unclear. Therefore, we prepared a fishing probe with conophylline to identify the target protein by using latex nano-beads, which are newly innovated tools for affinity-purification. With these conophylline-linked nano-beads, we found that conophylline directly interacted with ARL6IP. ARL6IP may thus be involved in the mechanism of cellular differentiation of β-cells, and this probe should be useful to find other target proteins.

本文言語English
ページ(範囲)6188-6195
ページ数8
ジャーナルBioorganic and Medicinal Chemistry
17
17
DOI
出版ステータスPublished - 2009 9月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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