To clarify the presence and origin of acetylcholine (ACh) in human blood, the ACh contents of whole blood, mononuclear (MNL) and polymorphonuclear leukocytes (PMN) were determined by a sensitive, specific radioimmunoassay. The mean (± S.E.M.) ACh contents of whole blood and MNL measured in 30 normal subjects were 1263.5 ± 149.0 and 731.0 ± 85.8 pg/ml blood, respectively. A significant correlation was observed between the ACh content of whole blood and that of MNL (r = 0.91; P < 0.001). No detectable ACh was present in PMN. These results demonstrate that ACh is present in human blood, and indicate that the major portion of plasma ACh originates from circulating MNL.
|出版ステータス||Published - 1993 1月 1|
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