TY - JOUR
T1 - Production of recombinant extracellular domains of canine desmoglein 1 (Dsg1) by baculovirus expression
AU - Nishifuji, Koji
AU - Amagai, Masayuki
AU - Nishikawa, Takeji
AU - Iwasaki, Toshiroh
N1 - Funding Information:
We wish to thank Dr. Susumu Tateyama for providing MCA-B1 cells. We are also grateful to Mrs. Yoshiko Fujii for cell culture and for preparing baculoproteins and to Mrs. Yuriko Okuno for help with nucleotide sequencing. This work is supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to T.I. and M.A.), and by a Health Sciences Research Grant for Research on Specific Diseases from the Ministry of Health, Labor and Welfare of Japan (to M.A.).
PY - 2003/10/15
Y1 - 2003/10/15
N2 - The aim of this study was to generate a recombinant protein to represent the entire extracellular domain of canine desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule, by the baculovirus expression system. Cotransfection of a baculovirus transfer vector containing the cDNA for the entire extracellular domain of canine Dsg1 with baculovirus DNA into insect cells resulted in the secretion of soluble canine Dsg1 into insect culture supernatants. Immunoreactivity of 11 human pemphigus foliaceus (PF) sera against the cell surface of canine keratinocytes was completely removed when the sera were preincubated with the canine Dsg1 baculoprotein. This recombinant canine Dsg1 produced by baculovirus shares the major epitopes of the authentic canine Dsg1 recognized by human PF sera, and will be useful in studying the molecular pathophysiological mechanisms in PF and impetigo in canine patients.
AB - The aim of this study was to generate a recombinant protein to represent the entire extracellular domain of canine desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule, by the baculovirus expression system. Cotransfection of a baculovirus transfer vector containing the cDNA for the entire extracellular domain of canine Dsg1 with baculovirus DNA into insect cells resulted in the secretion of soluble canine Dsg1 into insect culture supernatants. Immunoreactivity of 11 human pemphigus foliaceus (PF) sera against the cell surface of canine keratinocytes was completely removed when the sera were preincubated with the canine Dsg1 baculoprotein. This recombinant canine Dsg1 produced by baculovirus shares the major epitopes of the authentic canine Dsg1 recognized by human PF sera, and will be useful in studying the molecular pathophysiological mechanisms in PF and impetigo in canine patients.
KW - Baculovirus expression
KW - Desmoglein
KW - Dog
KW - Impetigo
KW - Pemphigus
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U2 - 10.1016/S0165-2427(03)00107-7
DO - 10.1016/S0165-2427(03)00107-7
M3 - Article
C2 - 12963278
AN - SCOPUS:0043237961
SN - 0165-2427
VL - 95
SP - 177
EP - 182
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 3-4
ER -