Protein Expression Micro-Scale Mapping Analysis of Endothelial Tissue in in Vitro Branched Vascular Model under Mechanical Stimuli

Jumpei Muramatsu, Azusa Shimizu, Michinao Hashimoto, Shigenori Miura, Hiroaki Onoe

研究成果: Conference contribution

抄録

This paper describes a micro-scale mapping of protein expression on endothelial tissue in an extracellular matrix (ECM)-based branched microchannel under mechanical stimuli. Our system mimics the heterogeneity in the distribution of complex mechanical stimuli applied to tissues in vivo by applying mechanical stimuli to a branched vascular model. We confirmed the up-regulation of gene expression levels in vascular endothelial cells(ECs) by stretch stress. We cultured ECs under complex mechanical stimuli, and visualized the distribution of mechanotransducer protein expression in the branched vascular model. We believe that our system could open a new door for the analysis of local cellular responses to mechanical stimuli.

本文言語English
ホスト出版物のタイトル35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
出版社Institute of Electrical and Electronics Engineers Inc.
ページ283-286
ページ数4
ISBN(電子版)9781665409117
DOI
出版ステータスPublished - 2022
イベント35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 - Tokyo, Japan
継続期間: 2022 1月 92022 1月 13

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
2022-January
ISSN(印刷版)1084-6999

Conference

Conference35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
国/地域Japan
CityTokyo
Period22/1/922/1/13

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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