TY - JOUR
T1 - Proteome expression database of ewing sarcoma
T2 - A segment of the genome medicine database of Japan proteomics
AU - Kikuta, Kazutaka
AU - Tsunehiro, Yukako
AU - Yoshida, Akihiko
AU - Tochigi, Naobumi
AU - Hirohahsi, Setsuo
AU - Kawai, Akira
AU - Kondo, Tadashi
PY - 2009
Y1 - 2009
N2 - Ewing sarcoma is the second most common primary malignant bone tumor in children and adolescents worldwide. Here, we report an open-access proteome expression database of eight Ewing sarcoma cases using proteome data obtained by two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. Proteins extracted from primary tumor tissues were labeled with CyDye DIGE Fluor saturation dye, and separated using a large format electrophoresis device, generating 2431 protein spots. Mass spectrometry following in-gel digestion identified 330 protein spots corresponding to 220 proteins. Multiple proteins were observed from single protein spots, and single proteins generated multiple protein spots, suggesting diversity of the proteome observed by 2D-DIGE. The results of 2D-DIGE and protein identification by mass spectrometry, and part of the corresponding clinico-pathological data such as prognosis after treatments are freely accessible in the public proteome database Genome Medicine Database of Japan Proteomics (GeMDBJ Proteomics, https://gemdbj.nibio.go.jp/dgdb/DigeTop.do).
AB - Ewing sarcoma is the second most common primary malignant bone tumor in children and adolescents worldwide. Here, we report an open-access proteome expression database of eight Ewing sarcoma cases using proteome data obtained by two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. Proteins extracted from primary tumor tissues were labeled with CyDye DIGE Fluor saturation dye, and separated using a large format electrophoresis device, generating 2431 protein spots. Mass spectrometry following in-gel digestion identified 330 protein spots corresponding to 220 proteins. Multiple proteins were observed from single protein spots, and single proteins generated multiple protein spots, suggesting diversity of the proteome observed by 2D-DIGE. The results of 2D-DIGE and protein identification by mass spectrometry, and part of the corresponding clinico-pathological data such as prognosis after treatments are freely accessible in the public proteome database Genome Medicine Database of Japan Proteomics (GeMDBJ Proteomics, https://gemdbj.nibio.go.jp/dgdb/DigeTop.do).
KW - Ewing sarcoma proteomics
KW - Gemdbj proteomics
KW - Mass spectrometry
KW - Proteome database
KW - Two-dimensional difference gel electrophoresis (2d-dige)
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U2 - 10.4172/jpb.1000112
DO - 10.4172/jpb.1000112
M3 - Article
AN - SCOPUS:73649107329
SN - 0974-276X
VL - 2
SP - 500
EP - 504
JO - Journal of Proteomics and Bioinformatics
JF - Journal of Proteomics and Bioinformatics
IS - 12
ER -