Proteomic and metabolomic analyses uncover sex-specific regulatory pathways in mouse fetal germline differentiation

Yohei Hayashi, Masaru Mori, Kaori Igarashi, Keiko Tanaka, Asuka Takehara, Yumi Ito-Matsuoka, Akio Kanai, Nobuo Yaegashi, Tomoyoshi Soga, Yasuhisa Matsui

研究成果: Article査読

6 被引用数 (Scopus)


Regulatory mechanisms of germline differentiation have generally been explained via the function of signaling pathways, transcription factors, and epigenetic regulation; however, little is known regarding proteomic and metabolomic regulation and their contribution to germ cell development. Here, we conducted integrated proteomic and metabolomic analyses of fetal germ cells in mice on embryonic day (E)13.5 and E18.5 and demonstrate sex- and developmental stage-dependent changes in these processes. In male germ cells, RNA processing, translation, oxidative phosphorylation, and nucleotide synthesis are dominant in E13.5 and then decline until E18.5, which corresponds to the prolonged cell division and more enhanced hyper-transcription/translation in male primordial germ cells and their subsequent repression. Tricarboxylic acid cycle and one-carbon pathway are consistently upregulated in fetal male germ cells, suggesting their involvement in epigenetic changes preceding in males. Increased protein stability and oxidative phosphorylation during female germ cell differentiation suggests an upregulation of aerobic energy metabolism, which likely contributes to the proteostasis required for oocyte maturation in subsequent stages. The features elucidated in this study shed light on the unrevealed mechanisms of germ cell development.

ジャーナルBiology of reproduction
出版ステータスPublished - 2020 10月 1

ASJC Scopus subject areas

  • 生殖医学
  • 細胞生物学


「Proteomic and metabolomic analyses uncover sex-specific regulatory pathways in mouse fetal germline differentiation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。