@article{5ba9770ee32c4e3697483d24e305578b,
title = "Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media",
abstract = "We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to Someya et al. (2021).",
keywords = "Cell culture, Metabolism, Stem Cells",
author = "Kotaro Kameda and Shota Someya and Jun Fujita and Keiichi Fukuda and Shugo Tohyama",
note = "Funding Information: The authors thank the Center for iPSC Research and Application and Kyoto University for providing the hiPSC lines (253G4 and FfI14s04). This work was mainly supported by the Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by the Japan Agency for Medical Research and Development (grant 20bm0404023h0003 to S.T.) and was partly supported by JSPS KAKENHI (grant 20H03768 to S.T.). Funding Information: The authors thank the Center for iPSC Research and Application and Kyoto University for providing the hiPSC lines (253G4 and FfI14s04). This work was mainly supported by the Projects for Technological Development, Research Center Network for Realization of Regenerative Medicine by the Japan Agency for Medical Research and Development (grant 20bm0404023h0003 to S.T.) and was partly supported by JSPS KAKENHI (grant 20H03768 to S.T.). K.K. performed experiments. K.K. S.S. and S.T. wrote the manuscript. J.F. K.F. and S.T. reviewed and edited the manuscript. S.T. J.F. and K.F. supervised this study. S.T. acquired funding. S.S. S.T. J.F. and K.F. have patents pending related to this study. K.F. is a cofounder and CEO of Heartseed Inc. S.T. is an advisor from Heartseed Inc. S.T. J.F. and K.F. own equity in Heartseed Inc. The remaining authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jun,
day = "17",
doi = "10.1016/j.xpro.2022.101341",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}