Protoplast fusion method for efficient transformation of mouse spermatogenic cells

Mitsuru Jimbo, Midori Matsumoto, Shun Ichi Kurata, Motonori Hoshi

研究成果: Article査読

4 被引用数 (Scopus)

抄録

The efficiencies of methods for introducing DNAs into spermatogenic cells were investigat ed. The calcium phosphate method has been most commonly used to transfect DNA into cultured cells, but this method severely damages spermatogenic cells. Therefore, we investigated the efficiencies of transfection of spermatogenic cells by DEAE-dextran, modified liposome, and protoplast fusion methods, which seemed to cause less damage to spermatogenic cells than the calcium phosphate method. The protoplast fusion method was the most suitable for transfection of spermatogenic cells.

本文言語English
ページ(範囲)321-323
ページ数3
ジャーナルJournal of biochemistry
117
2
DOI
出版ステータスPublished - 1995 2月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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