TY - JOUR
T1 - Purified opioid μ-receptor is of a different molecular size than δ- and κ-receptors
AU - Ueda, Hiroshi
AU - Harada, Hitoshi
AU - Misawa, Hidemi
AU - Nozaki, Masakatsu
AU - Takagi, Hiroshi
N1 - Funding Information:
We thank Ms, M. Ohara (Kyushu University, Japan) for reading the manuscript. Parts of this work were supported by a Grant-in-Aid for Scientific Research and Special Project Research from the Ministry of Education, Science and Culture, Japan and a Grant from the Naito Foundation.
PY - 1987/4/10
Y1 - 1987/4/10
N2 - Opioid μ-receptors were solubilized from rat brains with 0.5% Triton X-100 in the presence of 0.32 M sucrose and then purified with 6-succinyl morphine-Affi-Gel 102 column. Purified materials showed a major band with mol.wt. 58,000 Da and a minor band with mol.wt. 41,000 Da. Affinity cross linking with [3H]DAGO, a selective μ-opioid agonist, to the neural membranes and purified materials revealed that opioid μ-binding protein has a mol.wt. of 58,000 Da, a value totally different from that of δ- (mol.wt. 18,500 Da) and κ- (mol.wt. 36,600 Da) binding protein.
AB - Opioid μ-receptors were solubilized from rat brains with 0.5% Triton X-100 in the presence of 0.32 M sucrose and then purified with 6-succinyl morphine-Affi-Gel 102 column. Purified materials showed a major band with mol.wt. 58,000 Da and a minor band with mol.wt. 41,000 Da. Affinity cross linking with [3H]DAGO, a selective μ-opioid agonist, to the neural membranes and purified materials revealed that opioid μ-binding protein has a mol.wt. of 58,000 Da, a value totally different from that of δ- (mol.wt. 18,500 Da) and κ- (mol.wt. 36,600 Da) binding protein.
KW - 6-Succinyl morphine-Affi-Gel 102
KW - Cross linking
KW - Opioid μ-receptor
KW - Rat brain
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U2 - 10.1016/0304-3940(87)90546-5
DO - 10.1016/0304-3940(87)90546-5
M3 - Article
C2 - 2884604
AN - SCOPUS:0023152586
SN - 0304-3940
VL - 75
SP - 339
EP - 344
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -