@article{e0db422d8d054600a7f43725bb0e1f49,
title = "Quick-freeze, deep-etch visualization of the nuclear pore complex",
abstract = "We have visualized the nuclear pore complex (NPCx) three-dimensionally by quick-freeze, deep etching and suggest that NPCx is composed of four rings and associated filaments. The rings were located at the cytoplasmic side, at the nucleoplasmic side, and at the pore waist as well. Some of the filaments emanated toward the cytoplasm, some sprawled over the cytoplasmic face of the nuclear membrane, and some intervened the rings. The emanating filaments were considered to be vimentin filaments judging from the replica and thin-sectioned image.",
keywords = "Deep-etching, Intermediate filament, Nuclear membrane, Nuclear pore complex, Osteosarcoma, Quick-freeze",
author = "Youhei Fujitani and Shouzou Higaki and Hajime Sawada and Kazushige Hirosawa",
note = "Funding Information: Acknowledgments. We would like to thank Dr. A. Matsuzawa, Laboratory Animal Research Center, Institute of Medical Science, and Dr. R. Toyama, Department of Chemistry, Institute of Medical Science, for helpful advice. We would like to thank Ms. M. Inoue for typewriting, and also thank Ms. C. Ishiguro for advice on some English phrases. This work was supported by a Grant-in-Aid for Cancer Research (K. H. 61010024) from the Ministry of Education, Science and Culture, Japan.",
year = "1989",
language = "English",
volume = "38",
pages = "34--40",
journal = "Journal of Electron Microscopy",
issn = "0022-0744",
publisher = "Japanese Society of Microscopy",
number = "1",
}