@article{aa443a34439b40e6ba9b1760946c55d0,
title = "Radioimmunoassay for brain natriuretic peptide (BNP) detection of BNP in canine brain",
abstract = "We established a highly sensitive and specific radioimmunoassay (RIA) for BNP. Our RIA detected BNP-like immunoreactivity (-LI) in the porcine and canine brains but did not detect BNP-LI in the human, monkey or rat brain. The widespread distribution of BNP-LI was demonstrated both in the porcine and canine brains, with the highest concentration in the medulla oblongata. In contrast, the highest concentration of ANP-LI determined simultaneously was in the midbrain and the olfactory bulb. High performance-gel permeation chromatography coupled with RIA revealed that the major component of BNP-LI was eluted at the position of synthetic BNP with a small molecular weight (3K). These results indicate that the RIA for BNP serves as a useful tool to investigate physiological roles of BNP.",
author = "Hiroshi Itoh and Kazuwa Nakao and Yoshihiko Saito and Takayuki Yamada and Gotaro Shirakami and Masashi Mukoyama and Hiroshi Arai and Kiminori Hosoda and Suga, {Shin ichi} and Naoto Minamino and Kenji Kangawa and Hisayuki Matsuo and Hiroo Imura",
note = "Funding Information: We thank Ms Hiroko Fumon and Ms Atsuko Furu for the excellent secretarial assistance. This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare {"}Disorders of Adrenal Hormone{"} Research Committee, Japan, 1988, Life Science Research Project of Institute of Physical and Chemical Research (RIKEN), Japan Tobacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by research grants for cardiovascular diseases (62A'-I and 63C-2) from the Japanese Ministry of Health and Welfare.",
year = "1989",
month = jan,
day = "16",
doi = "10.1016/S0006-291X(89)80186-X",
language = "English",
volume = "158",
pages = "120--128",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",
}