Recognition of multiple epitopes in the human melanoma antigen gp100 by tumor-infiltrating T lymphocytes associated with in vivo tumor regression

Yutaka Kawakami, Siona Eliyahu, Christopher Jennings, Kazuyasu Sakaguchi, Xiaoqiang Kang, Scott Southwood, Paul F. Robbins, Alessandro Sette, Ettore Appella, Steven A. Rosenberg

研究成果: Article査読

535 被引用数 (Scopus)

抄録

Four often HLA-A2-restricted melanoma specific CTL that were derived from tumor-infiltrating lymphocytes (TIL) and administered to patients recognized the gp100 melanoma Ag and nine of ten recognized the MART-1 Ag. Adoptive transfer of the four gp100-reactive CTL, but not the other TIL, resulted in tumor regression when infused into autologous patients along with IL-2. Tumor regression was thus correlated with the recognition of gp100 by the administered T cells (p = 0.0048). To identify the epitopes recognized by these four gp100-reactive CTL, 169 peptides containing HLA-A2.1 binding motifs were synthesized and screened for their recognition by TIL using cytotoxicity and IFN-γ release assays. Five gp100 epitopes (two for TIL620, three for TIL660, one for TIL1143, and two for TIL1200) were recognized by CTL derived from different patients. Five of eight HLA-A2 binding melanoma epitopes (five gpl 00, one MART-1/Melan-A, two tyrosinase) had intermediate binding affinity to HLA-A2.1. These gp100 epitopes may be responsible for mediating tumor rejection in vivo and thus may be useful for the devel opment of immunotherapies for patients with melanoma.

本文言語English
ページ(範囲)3969-3974
ページ数6
ジャーナルJournal of Immunology
154
8
出版ステータスPublished - 1995
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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