Region dependent efficiency for recombinational transfer of the Bacillus subtilis 168 genome

Satoshi Tomita; Kenji Tsuge; Yo Kikuchi; Mitsuhiro Itaya

doi:10.1271/bbb.68.1382

Region dependent efficiency for recombinational transfer of the Bacillus subtilis 168 genome

Satoshi Tomita
, Kenji Tsuge
, Yo Kikuchi
, Mitsuhiro Itaya

研究成果: Article › 査読

7 被引用数 (Scopus)

抄録

Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shifted 98-kb region was targeted.

本文言語	English
ページ（範囲）	1382-1384
ページ数	3
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	68
号	6
DOI	https://doi.org/10.1271/bbb.68.1382
出版ステータス	Published - 2004 6月
外部発表	はい

ASJC Scopus subject areas

バイオテクノロジー
分析化学
生化学
応用微生物学とバイオテクノロジー
分子生物学
有機化学

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10.1271/bbb.68.1382

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abstract = "Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shifted 98-kb region was targeted.",

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AU - Tsuge, Kenji

AU - Kikuchi, Yo

AU - Itaya, Mitsuhiro

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Y1 - 2004/6

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AB - Submega-sized regions of the Bacillus subtilis genome were cloned to plasmid by the B. subtilis Recombinational Transfer (BReT) method. BReT efficiency depends not only on the genome location but also on the choice of sequences for simultaneous homologous recombination during BReT. In an extreme case, a 91-kb region that was unsuccessful on the first attempt was obtained when the slightly shifted 98-kb region was targeted.

KW - Giant plasmid

KW - Homologous recombination

KW - Landing pad sequence

KW - Recombinational transfer

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 6

ER -

Region dependent efficiency for recombinational transfer of the Bacillus subtilis 168 genome

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