@article{9bbd1e9c97d141a98227e1f6b23719c7,
title = "Reliability of urinary charged metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry",
abstract = "Currently, large-scale cohort studies for metabolome analysis have been launched globally. However, only a few studies have evaluated the reliability of urinary metabolome analysis. This study aimed to establish the reliability of urinary metabolomic profiling in cohort studies. In the Tsuruoka Metabolomics Cohort Study, 123 charged metabolites were identified and routinely quantified using capillary electrophoresis-mass spectrometry (CE-MS). We evaluated approximately 750 quality control (QC) samples and 6,720 participants{\textquoteright} spot urine samples. We calculated inter- and intra-batch coefficients of variation in the QC and participant samples and technical intraclass correlation coefficients (ICC). A correlation of metabolite concentrations between spot and 24-h urine samples obtained from 32 sub-cohort participants was also evaluated. The coefficient of variation (CV) was less than 20% for 87 metabolites (70.7%) and 20–30% for 19 metabolites (15.4%) in the QC samples. There was less than 20% inter-batch CV for 106 metabolites (86.2%). Most urinary metabolites would have reliability for measurement. The 96 metabolites (78.0%) was above 0.75 for the estimated ICC, and those might be useful for epidemiological analysis. Among individuals, the Pearson correlation coefficient of 24-h and spot urine was more than 70% for 59 of the 99 metabolites. These results show that the profiling of charged metabolites using CE-MS in morning spot human urine is suitable for epidemiological metabolomics studies.",
author = "Yoshiki Ishibashi and Sei Harada and Ayano Takeuchi and Miho Iida and Ayako Kurihara and Suzuka Kato and Kazuyo Kuwabara and Aya Hirata and Takuma Shibuki and Tomonori Okamura and Daisuke Sugiyama and Asako Sato and Kaori Amano and Akiyoshi Hirayama and Masahiro Sugimoto and Tomoyoshi Soga and Masaru Tomita and Toru Takebayashi",
note = "Funding Information: We thank the residents of Tsuruoka City for their interest in our study and the members of the Tsuruoka Metabo-lomic Cohort Study team for their commitment to the project. This study was supported in part by research funds from the Yamagata Prefectural Government (http://www.pref.yamagata.jp/) and the city of Tsuruoka (https://www.city.tsuruoka.lg.jp/), Grant-in-Aid for Scientific Research (B) (grant numbers JP24390168 and JP15H04778), Grant-in-Aid for Challenging Exploratory Research (grant number 25670303), and Grant-in-Aid for Scientific Research on Innovative Areas (grant number JP 16H06277) from the Japan Society for the Promotion of Science (http://www.jsps.go.jp/). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding Information: Ethical approval. This study was approved by the Medical Ethics Committee of the School of Medicine, Keio University, Tokyo, Japan (Approval No 20110264 and No 20130207 for the entire cohort study and the sub-cohort one, respectively). Informed consent was obtained in written form from all the participants included in the studies. All research was performed in accordance with the relevant guidelines and regulations. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41598-021-86600-9",
language = "English",
volume = "11",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}