Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii

Takashi Asai; Yasuhiro Suzuki

doi:10.1016/0378-1097(90)90350-Y

Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii

Takashi Asai, Yasuhiro Suzuki

研究成果: Article › 査読

19 被引用数 (Scopus)

抄録

Dithiothreitol-dependent MgATP² and MgADP^- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of V_max and K_m obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.

本文言語	English
ページ（範囲）	89-92
ページ数	4
ジャーナル	FEMS microbiology letters
巻	72
号	1-2
DOI	https://doi.org/10.1016/0378-1097(90)90350-Y
出版ステータス	Published - 1990 10月
外部発表	はい

ASJC Scopus subject areas

微生物学
分子生物学
遺伝学

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10.1016/0378-1097(90)90350-Y

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引用スタイル

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title = "Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii",

abstract = "Dithiothreitol-dependent MgATP2 and MgADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.",

keywords = "ADPase, ATPase, Nucleoside triphosphate hydrolase, Toxoplasma gondii",

author = "Takashi Asai and Yasuhiro Suzuki",

note = "Funding Information: Tb~s wor~, wa~ supported in part by Grant-in-Aid for Encouragement of Young Scientists 01770266 from the Ministry of Education, Science a,'~d Culture of Japan, and also supported in part by a grant from Ohm,a rea Health Foundation. We thank Dr. M. Kobayashi (Chiba University) 1or providing us a computer program for K,, and V,,,~ determination.",

year = "1990",

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doi = "10.1016/0378-1097(90)90350-Y",

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AU - Asai, Takashi

AU - Suzuki, Yasuhiro

N1 - Funding Information: Tb~s wor~, wa~ supported in part by Grant-in-Aid for Encouragement of Young Scientists 01770266 from the Ministry of Education, Science a,'~d Culture of Japan, and also supported in part by a grant from Ohm,a rea Health Foundation. We thank Dr. M. Kobayashi (Chiba University) 1or providing us a computer program for K,, and V,,,~ determination.

PY - 1990/10

Y1 - 1990/10

N2 - Dithiothreitol-dependent MgATP2 and MgADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.

AB - Dithiothreitol-dependent MgATP2 and MgADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically un tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.

KW - ADPase

KW - ATPase

KW - Nucleoside triphosphate hydrolase

KW - Toxoplasma gondii

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