Role of CD18-ICAM-1 in the entrapment of stimulated leukocytes in alveolar capillaries of perfused rat lungs

Takuya Aoki, Yukio Suzuki, Kazumi Nishio, Kouichi Suzuki, Atsusi Miyata, Yutaka Iigou, Hiroshi Serizawa, Harukuni Tsumura, Yuzuru Ishimura, Makoto Suematsu, Kazuhiro Yamaguchi

研究成果: Article査読

32 被引用数 (Scopus)


This study aimed to examine the behavior of stimulated leukocytes in the pulmonary microcirculation. The leukocyte-endothelium interaction was visualized under physiological shear rates in perfused rat lungs using high- speed confocal laser video microscopy. Leukocytes labeled with carboxyfluorescein were stimulated with cytokine-induced neutrophil chemoattractant (CINC/gro), which caused L-selectin shedding and inverse upregulation of CD18. Neither unstimulated nor stimulated leukocytes exhibited rolling in either pulmonary arterioles or venules, whereas both were sequestered in capillaries. Approximately 50% of stimulated leukocytes showed a transient cessation of movement in pulmonary capillaries. The CINC/gro stimulation, which inhibited leukocyte rolling and adhesion to mesenteric venules, reduced leukocyte velocity and increased leukocytes in pulmonary capillaries. Pretreatment with monoclonal antibodies against intercellular adhesion molecule-1 (ICAM-1) or CD18 attenuated these changes. Confocal microfluorography revealed constitutive expression of ICAM-1 not only in venules but also abundantly in capillary networks. These results suggest that selectin-independent, CD18-ICAM-1-dependent capillary sequestration is one of the major mechanisms by which activated leukocytes accumulate in the lungs.

ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
5 42-5
出版ステータスPublished - 1997

ASJC Scopus subject areas

  • 生理学
  • 循環器および心血管医学
  • 生理学(医学)


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