TY - JOUR
T1 - Role of mucoadhesive agent in ocular delivery of ganciclovir microemulsion
T2 - cytotoxicity evaluation in vitro and ex vivo
AU - Choudhari, Manisha
AU - Nayak, Kritika
AU - Nagai, Noriaki
AU - Nakazawa, Yosuke
AU - Khunt, Dignesh
AU - Misra, Manju
N1 - Funding Information:
The corresponding author would like to thank, Department of Science and Technology and SERB (INSPIRE Grant No: IFA-LSBM-13 and EMR/2016/007966/HS) for project funds and Ministry of Chemicals and Fertilizer for providing fellowship.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2023/4
Y1 - 2023/4
N2 - Purpose: The aim of the present study was to investigate increase in delivery of drug upon formulation as mucoadhesive microemulsion system and further to investigate possibility of any cytotoxic effects using such formulation. Material and methods: Considering hydrophilic and small molecular nature of the drug, it was attempted to be formulated as microemulsion, by using pseudo ternary phase diagram method. Thus, three types of microemulsions were prepared; oil in water, water in oil type and chitosan-coated microemulsion. These microemulsions were characterized for several physicochemical properties like size, zeta potential, Polydispersity index, and compared for in vitro cell viability and ex vivo corneal irritation study. Results: All three microemulsions were quite stable, transparent and homogenous systems. They showed similar drug release pattern, but highest ex vivo goat corneal permeation was observed with Chitosan coated microemulsion when compared with ganciclovir solution. Conclusion: All microemulsions were found to be non-irritant in in vitro cell viability assay and ex vivo corneal irritation study, indicating the potential of using such systems for delivery of drug to eye.
AB - Purpose: The aim of the present study was to investigate increase in delivery of drug upon formulation as mucoadhesive microemulsion system and further to investigate possibility of any cytotoxic effects using such formulation. Material and methods: Considering hydrophilic and small molecular nature of the drug, it was attempted to be formulated as microemulsion, by using pseudo ternary phase diagram method. Thus, three types of microemulsions were prepared; oil in water, water in oil type and chitosan-coated microemulsion. These microemulsions were characterized for several physicochemical properties like size, zeta potential, Polydispersity index, and compared for in vitro cell viability and ex vivo corneal irritation study. Results: All three microemulsions were quite stable, transparent and homogenous systems. They showed similar drug release pattern, but highest ex vivo goat corneal permeation was observed with Chitosan coated microemulsion when compared with ganciclovir solution. Conclusion: All microemulsions were found to be non-irritant in in vitro cell viability assay and ex vivo corneal irritation study, indicating the potential of using such systems for delivery of drug to eye.
KW - Cell viability assay
KW - Chitosan
KW - Ganciclovir
KW - Microemulsions
KW - Mucoadhesive
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U2 - 10.1007/s10792-022-02514-z
DO - 10.1007/s10792-022-02514-z
M3 - Article
C2 - 36156180
AN - SCOPUS:85138757663
SN - 0165-5701
VL - 43
SP - 1153
EP - 1167
JO - International Ophthalmology
JF - International Ophthalmology
IS - 4
ER -