TY - JOUR
T1 - Selective killing of fibroblast-like cells in cultures of mouse heart cells by treatment with a Ca ionophore, A23187
AU - Kaneko, Hiroyuki
AU - Goshima, Kiyota
N1 - Funding Information:
We thank ProfessorJ . Asai for his kind suggestionso n the terminology of morphological degeneration of fibroblast-like cells and to Dr S. Wakabayashif or his heluful advice on the ‘Ta uotake experiments.T his work was supported by grants (to i<.G.) from the Ministry of Education, Science and Culture, Japan, and the-IshidaS cience Foundation.
PY - 1982/12
Y1 - 1982/12
N2 - Myocardial cells uncontaminated by fibroblast-like cells could be obtained by treating mouse heart cell cultures with a Ca ionophore, A23187. When trypsin-dissociated mouse heart cells were cultivated for 1 day or more on Petri dishes, both myocardial and fibroblast-like cells were detected on the dish under a phase contrast microscope. However, when the cultured heart cells were treated for a short period (e.g. 10 min) at 25 °C with A23187, only fibroblast-like cells exhibited morphological degeneration, such as nuclear pycnosis, cytoplasmic condensation and bleb- and balloon-formation. The myocardial cells maintained their normal morphology during the treatment; transient irregular beating was observed, but disappeared after removal of A23187. Thus treatment of heart cell cultures with A23187 can yield a large amount of myocardial cells without contamination by fibroblast-like cells. The possible mechanism for this selective effect is discussed.
AB - Myocardial cells uncontaminated by fibroblast-like cells could be obtained by treating mouse heart cell cultures with a Ca ionophore, A23187. When trypsin-dissociated mouse heart cells were cultivated for 1 day or more on Petri dishes, both myocardial and fibroblast-like cells were detected on the dish under a phase contrast microscope. However, when the cultured heart cells were treated for a short period (e.g. 10 min) at 25 °C with A23187, only fibroblast-like cells exhibited morphological degeneration, such as nuclear pycnosis, cytoplasmic condensation and bleb- and balloon-formation. The myocardial cells maintained their normal morphology during the treatment; transient irregular beating was observed, but disappeared after removal of A23187. Thus treatment of heart cell cultures with A23187 can yield a large amount of myocardial cells without contamination by fibroblast-like cells. The possible mechanism for this selective effect is discussed.
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U2 - 10.1016/0014-4827(82)90382-2
DO - 10.1016/0014-4827(82)90382-2
M3 - Article
C2 - 6816622
AN - SCOPUS:0020352452
SN - 0014-4827
VL - 142
SP - 407
EP - 416
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -