抄録
The Min system determines the cell division plane of bacteria. As a cue of spatiotemporal regulation, the Min system uses wave propagation of MinD protein (Min wave). Therefore, the reconstitution of the Min wave in cell-sized closed space will lead to the creation of artificial cells capable of cell division. The Min waves emerge via coupling between the reactions among MinD, MinE, and ATP and the differences in diffusion rate on the cell membrane and in the cytoplasm. Because Min waves appear only under the balanced condition of the reaction-diffusion coupling, special attentions are needed towards several technical points for the reconstitution of Min waves in artificial cells. This protocol describes a technical method for stably generating Min waves in artificial cells.
| 本文言語 | English |
|---|---|
| 論文番号 | e3561 |
| ジャーナル | Bio-protocol |
| 巻 | 10 |
| 号 | 6 |
| DOI | |
| 出版ステータス | Published - 2020 3月 20 |
ASJC Scopus subject areas
- 神経科学（全般）
- 生化学、遺伝学、分子生物学（全般）
- 免疫学および微生物学（全般）
- 植物科学