TY - JOUR
T1 - Sequence of the EndoA gene encoding mouse cytokeratin and its methylation state in the CpG-rich region
AU - Tamai, Yoshitaka
AU - Takemoto, Yoshihiro
AU - Matsumoto, Midori
AU - Morita, Takashi
AU - Matsushiro, Aizo
AU - Nozaki, Masami
PY - 1991/8/15
Y1 - 1991/8/15
N2 - A genomic clone obtained from mouse liver DNA using a mouse cytokeratin EndoA cDNA probe revealed the complete sequence of the EndoA gene. The gene is divided into nine exons and the exon-intron pattern has been conserved compared to that of other type-II cytokeratin-encoding genes. The 5' upstream, 3' downstream and first and third introns contain potential regulatory sequences, including polyoma virus enhancer motifs (PEA1 and PEA3) and AP-1 elements. The 5' regions upstream of the EndoA EndoB and Ck8 genes contain homologous sequences surrounding the TATA boxes. In addition, a CpG dinucleotide cluster region was located around the first exon. This CpG cluster region was found to be hypomethylated in endodermal PYS-2 cells, retinoic acid-treated F9 cells, and F9 embryonal carcinoma cells, but hypermethylated in BALB/C 3T3 fibroblast cells that do not express EndoA. These findings may provide a clue to understanding the molecular mechanisms of EndoA gene expression.
AB - A genomic clone obtained from mouse liver DNA using a mouse cytokeratin EndoA cDNA probe revealed the complete sequence of the EndoA gene. The gene is divided into nine exons and the exon-intron pattern has been conserved compared to that of other type-II cytokeratin-encoding genes. The 5' upstream, 3' downstream and first and third introns contain potential regulatory sequences, including polyoma virus enhancer motifs (PEA1 and PEA3) and AP-1 elements. The 5' regions upstream of the EndoA EndoB and Ck8 genes contain homologous sequences surrounding the TATA boxes. In addition, a CpG dinucleotide cluster region was located around the first exon. This CpG cluster region was found to be hypomethylated in endodermal PYS-2 cells, retinoic acid-treated F9 cells, and F9 embryonal carcinoma cells, but hypermethylated in BALB/C 3T3 fibroblast cells that do not express EndoA. These findings may provide a clue to understanding the molecular mechanisms of EndoA gene expression.
KW - AP-1
KW - Genomic DNA
KW - exon-intron
KW - intermediate filament
KW - polyoma enhancer
KW - teratocarcinoma
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U2 - 10.1016/0378-1119(91)90247-9
DO - 10.1016/0378-1119(91)90247-9
M3 - Article
C2 - 1717348
AN - SCOPUS:0025936469
SN - 0378-1119
VL - 104
SP - 169
EP - 176
JO - Gene
JF - Gene
IS - 2
ER -