Sequential insertion of multiple I-SceI recognition sites at designed loci of the bacillus subtilis 168 genome

Mitsuhiro Itaya

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives.

本文言語English
ページ(範囲)180-182
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
76
1
DOI
出版ステータスPublished - 2012
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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