TY - GEN
T1 - Sequential production of various types of asymmetric lipid vesicles using pulse jet flow
AU - Gotanda, Masahide
AU - Kamiya, Kaki
AU - Osaki, Toshihisa
AU - Fujii, Satoshi
AU - Misawa, Nobuo
AU - Miki, Norihisa
AU - Takeuchi, Shoji
N1 - Funding Information:
We thank Yoshimi Nozaki for technical assistance in arranging experimental laboratory. This work was partly supported by JSPS KAKENHI (Young Scientists (A)) Grant Number 15H05493 (K. K.), the Regional Innovation Strategy Support Program of MEXT, and ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan).
Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/26
Y1 - 2017/7/26
N2 - This paper describes a method of sequential formation of asymmetric lipid vesicles, which applies pulse jet flow to an asymmetric planar lipid bilayer with multiple lipid components formed by Droplet Split-and-Contact Method. In our previous work, we generated asymmetric lipid vesicles by deforming an asymmetric planar lipid bilayer using a pulsed jet flow. However, it was difficult to create various types or large amounts of the asymmetric lipid vesicles in a single double-well device. In this study, asymmetric planar lipid bilayers with multiple lipid components were formed by contacting various types of lipid monolayers using movable wells on a revolving table. We successfully produced two types of the asymmetric lipid vesicles containing red or green fluorescent lipids on the outer leaflet with a single device in a high-throughput manner using the proposed method. The asymmetricity of the generated vesicles was evaluated by the distribution of the fluorescence intensities. The proposed device will be applicable for generating artificial cell models with multiple asymmetric lipid components.
AB - This paper describes a method of sequential formation of asymmetric lipid vesicles, which applies pulse jet flow to an asymmetric planar lipid bilayer with multiple lipid components formed by Droplet Split-and-Contact Method. In our previous work, we generated asymmetric lipid vesicles by deforming an asymmetric planar lipid bilayer using a pulsed jet flow. However, it was difficult to create various types or large amounts of the asymmetric lipid vesicles in a single double-well device. In this study, asymmetric planar lipid bilayers with multiple lipid components were formed by contacting various types of lipid monolayers using movable wells on a revolving table. We successfully produced two types of the asymmetric lipid vesicles containing red or green fluorescent lipids on the outer leaflet with a single device in a high-throughput manner using the proposed method. The asymmetricity of the generated vesicles was evaluated by the distribution of the fluorescence intensities. The proposed device will be applicable for generating artificial cell models with multiple asymmetric lipid components.
KW - Artificial Cell Membrane
KW - Asymmetric lipid bilayer
KW - Asymmetric lipid vesicles
KW - Liposome
UR - http://www.scopus.com/inward/record.url?scp=85029375738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029375738&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2017.7994113
DO - 10.1109/TRANSDUCERS.2017.7994113
M3 - Conference contribution
AN - SCOPUS:85029375738
T3 - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 572
EP - 574
BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Y2 - 18 June 2017 through 22 June 2017
ER -