TY - JOUR
T1 - Serum anti-AP3D1 antibodies are risk factors for acute ischemic stroke related with atherosclerosis
AU - Li, Shu Yang
AU - Yoshida, Yoichi
AU - Kobayashi, Eiichi
AU - Kubota, Masaaki
AU - Matsutani, Tomoo
AU - Mine, Seiichiro
AU - Machida, Toshio
AU - Maezawa, Yoshiro
AU - Takemoto, Minoru
AU - Yokote, Koutaro
AU - Kobayashi, Yoshio
AU - Takizawa, Hirotaka
AU - Sata, Mizuki
AU - Yamagishi, Kazumasa
AU - Iso, Hiroyasu
AU - Sawada, Norie
AU - Tsugane, Shoichiro
AU - Kobayashi, Sohei
AU - Matsushita, Kazuyuki
AU - Nomura, Fumio
AU - Matsubara, Hisahiro
AU - Sumazaki, Makoto
AU - Ito, Masaaki
AU - Yajima, Satoshi
AU - Shimada, Hideaki
AU - Iwase, Katsuro
AU - Ashino, Hiromi
AU - Wang, Hao
AU - Goto, Kenichiro
AU - Tomiyoshi, Go
AU - Shinmen, Natsuko
AU - Nakamura, Rika
AU - Kuroda, Hideyuki
AU - Iwadate, Yasuo
AU - Hiwasa, Takaki
N1 - Funding Information:
The present study was supported, in part, by research Grants from JSPS KAKENHI Grant Numbers 20K17953, 19K09451, 17K19810, 20K07810, 16K10520, 15K10117, and 15K01842, the Japan Agency for Medical Research and Development (AMED), and Japan Science and Technology Agency (JST).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Atherosclerosis has been considered as the main cause of morbidity, mortality, and disability worldwide. The first screening for antigen markers was conducted using the serological identification of antigens by recombinant cDNA expression cloning, which has identified adaptor-related protein complex 3 subunit delta 1 (AP3D1) as an antigen recognized by serum IgG antibodies of patients with atherosclerosis. Serum antibody levels were examined using the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) using a recombinant protein as an antigen. It was determined that the serum antibody levels against AP3D1 were higher in patients with acute ischemic stroke (AIS), transient ischemic attack, diabetes mellitus (DM), cardiovascular disease, chronic kidney disease (CKD), esophageal squamous cell carcinoma (ESCC), and colorectal carcinoma than those in the healthy donors. The area under the curve values of DM, nephrosclerosis type of CKD, and ESCC calculated using receiver operating characteristic curve analysis were higher than those of other diseases. Correlation analysis showed that the anti-AP3D1 antibody levels were highly associated with maximum intima-media thickness, which indicates that this marker reflected the development of atherosclerosis. The results of the Japan Public Health Center-based Prospective Study indicated that this antibody marker is deemed useful as risk factors for AIS.
AB - Atherosclerosis has been considered as the main cause of morbidity, mortality, and disability worldwide. The first screening for antigen markers was conducted using the serological identification of antigens by recombinant cDNA expression cloning, which has identified adaptor-related protein complex 3 subunit delta 1 (AP3D1) as an antigen recognized by serum IgG antibodies of patients with atherosclerosis. Serum antibody levels were examined using the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) using a recombinant protein as an antigen. It was determined that the serum antibody levels against AP3D1 were higher in patients with acute ischemic stroke (AIS), transient ischemic attack, diabetes mellitus (DM), cardiovascular disease, chronic kidney disease (CKD), esophageal squamous cell carcinoma (ESCC), and colorectal carcinoma than those in the healthy donors. The area under the curve values of DM, nephrosclerosis type of CKD, and ESCC calculated using receiver operating characteristic curve analysis were higher than those of other diseases. Correlation analysis showed that the anti-AP3D1 antibody levels were highly associated with maximum intima-media thickness, which indicates that this marker reflected the development of atherosclerosis. The results of the Japan Public Health Center-based Prospective Study indicated that this antibody marker is deemed useful as risk factors for AIS.
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U2 - 10.1038/s41598-021-92786-9
DO - 10.1038/s41598-021-92786-9
M3 - Article
C2 - 34188129
AN - SCOPUS:85108960829
SN - 2045-2322
VL - 11
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 13450
ER -
