Serum response factor is modulated by the SUMO-1 conjugation system

Kazuhito Matsuzaki, Takeshi Minami, Masahide Tojo, Yoshiomi Honda, Yasuhiro Uchimura, Hisato Saitoh, Hideyo Yasuda, Shinji Nagahiro, Hideyuki Saya, Mitsuyoshi Nakao

研究成果: Article査読

45 被引用数 (Scopus)


Serum stimulation leads to activation of the serum response factor (SRF)-mediated transcription of immediate-early genes such as c-fos via various signal transduction pathways. We have previously reported that promyelocytic leukemia protein (PML) is involved in the transcriptional regulation by SRF. PML is one of the well-known substrates for modification by small ubiquitin-related modifier-1 (SUMO-1) and several SUMO-1-modified proteins associate with PML. Here, we report that SRF is modified by SUMO-1 chiefly at lysine147 within the DNA-binding domain. Substitution of this target lysine for alanine did not affect the translocation of SRF to PML-nuclear bodies. The SRF mutant augmented the transcriptional activity under Rho A-stimulated condition but not under serum-starved condition, suggesting that activated SRF is suppressed by its sumoylation. These data support the transcriptional role of SUMO-1 conjugating system in cellular serum response.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータスPublished - 2003 6月 20

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


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