TY - JOUR
T1 - "Smart Eye Camera"
T2 - An innovative technique to evaluate tear film breakup time in a murine dry eye disease model
AU - Shimizu, Eisuke
AU - Ogawa, Yoko
AU - Yazu, Hiroyuki
AU - Aketa, Naohiko
AU - Yang, Fan
AU - Yamane, Mio
AU - Sato, Yasunori
AU - Kawakami, Yutaka
AU - Tsubota, Kazuo
N1 - Funding Information:
This work was partially supported by the Japanese Ministry of Education, Science, Sports, Culture, and Technology, #18K09421 and The Uehara Memorial Foundation. No other funding statement to declare. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The OUI Inc. supported in providing the device for authors [insert relevant initials], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank Dr. Shigeru Nakamura, Dr. Toshihiro Imada, Dr. Takashi Kojima, and Dr. Shizuo Mukai for their valuable advice.
Publisher Copyright:
© 2019 Shimizu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2019/5
Y1 - 2019/5
N2 - Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and is capable of evaluating TFBUT in a murine DED model. We compared images taken by existing devices and the Smart Eye Camera in a graft-versus-host disease-related DED murine model. We observed that the quality of the images obtained by the Smart Eye Camera were sufficient for practical use. Moreover, this new technique could be used to obtain measurements for several consecutive ocular phenotypes in a variety of environments. Here, we demonstrate the effectiveness of our new invention in the examination of ocular phenotypes, including TFBUT in a murine model. We highlight the potential for future translational studies adopting the Smart Eye Camera in clinical settings.
AB - Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet to be established. We invented an innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and is capable of evaluating TFBUT in a murine DED model. We compared images taken by existing devices and the Smart Eye Camera in a graft-versus-host disease-related DED murine model. We observed that the quality of the images obtained by the Smart Eye Camera were sufficient for practical use. Moreover, this new technique could be used to obtain measurements for several consecutive ocular phenotypes in a variety of environments. Here, we demonstrate the effectiveness of our new invention in the examination of ocular phenotypes, including TFBUT in a murine model. We highlight the potential for future translational studies adopting the Smart Eye Camera in clinical settings.
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U2 - 10.1371/JOURNAL.PONE.0215130
DO - 10.1371/JOURNAL.PONE.0215130
M3 - Article
C2 - 31071120
AN - SCOPUS:85065654187
SN - 1932-6203
VL - 14
JO - PloS one
JF - PloS one
IS - 5
M1 - e0215130
ER -