Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. Both amine ends of a poly(ethylene oxide) (PEO) (M.W.5000) were allowed to react with a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS), which can specifically bind to an integrin receptor. Large cell agglomerates could be readily and soon obtained by adding GRGDS-PEO-GRGDS chains to a cell suspension and subsequently centrifugating cells. The agglomerated cells adhered onto the collagen-coated PS plate and then cells spread and migrated. Eventually, cell shapes changed from agglomerates to a monolayer. Moreover, we could observed that cells tended to attach each other closer than the conventional cell seeding.
|出版ステータス||Published - 2005 12月 1|
|イベント||54th SPSJ Annual Meeting 2005 - Yokohama, Japan|
継続期間: 2005 5月 25 → 2005 5月 27
|Other||54th SPSJ Annual Meeting 2005|
|Period||05/5/25 → 05/5/27|
ASJC Scopus subject areas