@article{5904600e87dd4f57b84be605edd11112,
title = "Synaptic Specializations of Melanopsin-Retinal Ganglion Cells in Multiple Brain Regions Revealed by Genetic Label for Light and Electron Microscopy",
abstract = "The form and synaptic fine structure of melanopsin-expressing retinal ganglion cells, also called intrinsically photosensitive retinal ganglion cells (ipRGCs), were determined using a new membrane-targeted version of a genetic probe for correlated light and electron microscopy (CLEM). ipRGCs project to multiple brain regions, and because the method labels the entire neuron, it was possible to analyze nerve terminals in multiple retinorecipient brain regions, including the suprachiasmatic nucleus (SCN), olivary pretectal nucleus (OPN), and subregions of the lateral geniculate. Although ipRGCs provide the only direct retinal input to the OPN and SCN, ipRGC terminal arbors and boutons were found to be remarkably different in each target region. A network of dendro-dendritic chemical synapses (DDCSs) was also revealed in the SCN, with ipRGC axon terminals preferentially synapsing on the DDCS-linked cells. The methods developed to enable this analysis should propel other CLEM studies of long-distance brain circuits at high resolution.",
keywords = "IGL, LGN, OPN, SCN, circadian, ipRGC, mRGC, melanopsin, miniSOG, serial blockface electron microscopy",
author = "Kim, {Keun Young} and Rios, {Luis C.} and Hiep Le and Perez, {Alex J.} and S{\'e}bastien Phan and Bushong, {Eric A.} and Deerinck, {Thomas J.} and Liu, {Yu Hsin} and Ellisman, {Maya A.} and Varda Lev-Ram and Suyeon Ju and Panda, {Sneha A.} and Sanghee Yoon and Masatoshi Hirayama and Mure, {Ludovic S.} and Megumi Hatori and Ellisman, {Mark H.} and Satchidananda Panda",
note = "Funding Information: The research was supported by NIH NEI grant EY 016807 (to S.P.), NIH NIGMS P41GM103412 and RO1 GM086197 (to M.H.E.), and NINDS RO1 NS027177 (to M.H.E.). We also received support from NIH R01GM086197 . We thank Dr. David O{\textquoteright}Keefe for help with preparing the manuscript and Daniela Boassa, Hiroyuki Hakozaki, Willy Wong, and Andrea Thor for technical support. Funding Information: The research was supported by NIH NEI grant EY 016807 (to S.P.), NIH NIGMS P41GM103412 and RO1 GM086197 (to M.H.E.), and NINDS RO1 NS027177 (to M.H.E.). We also received support from NIH R01GM086197. We thank Dr. David O'Keefe for help with preparing the manuscript and Daniela Boassa, Hiroyuki Hakozaki, Willy Wong, and Andrea Thor for technical support. M.H.E. played a key role in SBEM data collection for the project, and S. Panda played a key role in the biology and data analyses of this project. Quantitative Data Analyses, K.-Y.K. L.C.R. A.J.P. and S. Phan; Resources, M.H.E. and S. Panda; Validation, H.L. M. Hatori, V.L.-R. M.A.E. and A.J.P.; Investigation, K.-Y.K. L.C.R. Y.H.L. A.J.P. L.S.M. S. Phan, E.A.B. S.J. S.A.P. M. Hirayama, S.Y. and S. Panda; Visualization, K.-Y.K. L.C.R. Y.H.L. and A.J.P.; Methodology, K.-Y.K. S.P. E.A.B. T.J.D. and M.H.E.; Writing ? Original Draft, K.-Y.K. L.C.R. Y.H.L. S. Panda, M.H.E.; Writing, K.-Y.K. L.C.R. M.H.E. and S. Panda; Review and Editing, K.-Y.K. L.C.R. T.J.D. E.A.B. M.H.E. and S. Panda. S. Panda is the author of the book ?The Circadian Code.? Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = oct,
day = "15",
doi = "10.1016/j.celrep.2019.09.006",
language = "English",
volume = "29",
pages = "628--644.e6",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "3",
}