Target DNA detection by solid-state nanopores using gold nanoparticle probes

Rena Akahori, Keiko Esashika, Itaru Yanagi, Toshiharu Saiki

研究成果: Article査読

2 被引用数 (Scopus)


For target DNA detection with nanopores, the labeling of target DNA with large probes and the detection of its passage through a nanopore were examined. We labeled the target DNA with two gold nanoparticles (AuNPs). The surface of each AuNP was modified with a different single-stranded DNA (ssDNA) molecule that contained a complementary sequence to a different part of the target DNA so that the two AuNPs could capture one target DNA. The observed ionic current blockade when the AuNPs-labeled target DNA passed through a nanopore was significantly larger than that when nonlabeled DNA or a single AuNP probe passed through a nanopore. This result indicated that the presence of the target DNA in solution can be detected through nanopore measurements with two AuNP probes.

ジャーナルJapanese journal of applied physics
出版ステータスPublished - 2020 10月 1

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)


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