@article{7bbd1ff2a1b8418598f4a83c1f4bc2f7,
title = "TCR sequencing analysis of cancer tissues and tumor draining lymph nodes in colorectal cancer patients",
abstract = "Tumor draining lymph nodes (TDLNs) are located in the routes of lymphatic drainage from a primary tumor and have the highest risk of metastasis in various types of solid tumors. TDLNs are also considered as a tissue to activate the antitumor immunity, where antigen-specific effector T cells are generated. However, T cell receptor (TCR) repertoires in TDLNs have not been well characterized. We collected 23 colorectal cancer tumors with 203 lymph nodes with/without metastatic cancer cells (67 were metastasis-positive and the remaining 136 were metastasis-negative) and performed TCR sequencing. Metastasis-positive TDLNs showed a significantly lower TCR diversity and shared TCR clonotypes more frequently with primary tumor tissues compared to metastasis-negative TDLNs. Principal component analysis indicated that TDLNs with metastasis showed similar TCR repertoires. These findings suggest that cancer-reactive T cell clones could be enriched in the metastasis-positive TDLNs.",
keywords = "T cell receptor, colorectal cancer, immunogenomics, immunotherapy, lymph node",
author = "Tatsuo Matsuda and Eisaku Miyauchi and Hsu, {Yu Wen} and Satoshi Nagayama and Kazuma Kiyotani and Makda Zewde and Park, {Jae Hyun} and Taigo Kato and Makiko Harada and Shimpei Matsui and Masashi Ueno and Kazumasa Fukuda and Nobuaki Suzuki and Shoichi Hazama and Hiroaki Nagano and Hiroya Takeuchi and Vigneswaran, {Wickii T.} and Yuko Kitagawa and Yusuke Nakamura",
note = "Funding Information: This work was supported by the OncoTherapy Science Inc. We appreciate Drs. Rui Yamaguchi, Seiya Imoto and Satoru Miyano at the University of Tokyo for developing the algorithm of TCR repertoire analysis and helpful support in data management. The super-computing resource (http://sc.hgc.jp/shirokane.html) was provided by Human Genome Center, the Institute of Medical Science, the University of Tokyo. We also thank Yuki Hirata, Masashi Takeuchi and Adrian Rodrigues for excellent help for sample collections. Funding Information: aDepartment of Medicine, The University of Chicago, Chicago, IL, USA; bDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan; cDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan; dThe PhD Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei, Taiwan; eDepartment of Gastroenterological Surgery, Gastroenterological Center, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan; fCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan; gDepartment of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan; hDepartment of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan; iDepartment of Thoracic and Cardiovascular Surgery, Loyola University Medical Center, Maywood, IL, USA Funding Information: This work was supported by the OncoTherapy Science Inc. Publisher Copyright: {\textcopyright} 2019, {\textcopyright} 2019 Taylor & Francis Group, LLC.",
year = "2019",
month = jun,
day = "3",
doi = "10.1080/2162402X.2019.1588085",
language = "English",
volume = "8",
journal = "OncoImmunology",
issn = "2162-4011",
publisher = "Landes Bioscience",
number = "6",
}
