TY - JOUR
T1 - The possible role of apoptosis-suppressing genes, bcl-2 and mcl-1/EAT in human adrenal tumors
AU - Ando, Takashi
AU - Shibata, Hirotaka
AU - Suzuki, Toshihiko
AU - Kurihara, Isao
AU - Hayashi, Kouichi
AU - Hayashi, Matsuhiko
AU - Saito, Ikuo
AU - Kawabe, Hiroshi
AU - Tsujioka, Minako
AU - Saruta, Takao
N1 - Funding Information:
The authors thank M. Murai and S. Baba of the Department of Urology, School of Medicine, Keio University for kindly providing adrenal tissues. We also thank Dr. Umezawa for providing cDNA probes. This work was supported by Keio Medical Society, and High-Tech research center.
PY - 1998
Y1 - 1998
N2 - The expression levels of bcl-2, mcl-1/EAT, and bax were examined by Northern blot analysis and semi-quantitative RT-PCR method in 25 adrenal tumors, including seven adrenal pheochromocytomas (PHE), seven aldosterone- producing adenomas (APA), four adrenal cortisol-producing adenomas (CS), one deoxycorticosterone-producing adenoma (DOC) and six non-hyperfunctioning adrenal cortical adenomas (NF). Northern blot analysis revealed both bcl-2 and mcl-1/EAT mRNAs in all of the adrenal tumors. The expression levels differed greatly among the tumor samples. Mcl-1/EAT mRNA levels were enhanced in APA and CS compared with those in NF. In contrast, bcl-2 levels in NF were higher than in other tumors. Our results suggest that deregulation of such apoptosis-suppressing genes may contribute to the multistep tumorigenesis of human adrenal tumors and that mcl-1/EAT, one of the bcl-2 family genes, has a different role from that of bcl-2 in such pathways.
AB - The expression levels of bcl-2, mcl-1/EAT, and bax were examined by Northern blot analysis and semi-quantitative RT-PCR method in 25 adrenal tumors, including seven adrenal pheochromocytomas (PHE), seven aldosterone- producing adenomas (APA), four adrenal cortisol-producing adenomas (CS), one deoxycorticosterone-producing adenoma (DOC) and six non-hyperfunctioning adrenal cortical adenomas (NF). Northern blot analysis revealed both bcl-2 and mcl-1/EAT mRNAs in all of the adrenal tumors. The expression levels differed greatly among the tumor samples. Mcl-1/EAT mRNA levels were enhanced in APA and CS compared with those in NF. In contrast, bcl-2 levels in NF were higher than in other tumors. Our results suggest that deregulation of such apoptosis-suppressing genes may contribute to the multistep tumorigenesis of human adrenal tumors and that mcl-1/EAT, one of the bcl-2 family genes, has a different role from that of bcl-2 in such pathways.
UR - http://www.scopus.com/inward/record.url?scp=0032446757&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032446757&partnerID=8YFLogxK
U2 - 10.3109/07435809809032715
DO - 10.3109/07435809809032715
M3 - Article
C2 - 9888605
AN - SCOPUS:0032446757
SN - 0743-5800
VL - 24
SP - 955
EP - 960
JO - Endocrine Research
JF - Endocrine Research
IS - 3-4
ER -