TY - JOUR
T1 - The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in whole-blood samples
AU - Obara, Hideaki
AU - Aikawa, Naoki
AU - Hasegawa, Naoki
AU - Hori, Shingo
AU - Ikeda, Yasuo
AU - Kobayashi, Yoshio
AU - Murata, Mitsuru
AU - Okamoto, Shinichiro
AU - Takeda, Junzo
AU - Tanabe, Minoru
AU - Sakakura, Yasuhiko
AU - Ginba, Hiroyuki
AU - Kitajima, Masaki
AU - Kitagawa, Yuko
N1 - Funding Information:
Acknowledgments This work was partially supported by Roche Diagnostics. The authors received research funding, reagents, and equipment from Roche Diagnostics for this study.
PY - 2011/6
Y1 - 2011/6
N2 - The rapid diagnosis of pathogens and prompt initiation of appropriate antibiotic therapy are critical factors to reduce the morbidity and mortality associated with sepsis. In this study, we evaluated a multiplex polymerase chain reaction (PCR-M) test that detects bacteria and fungi in whole-blood specimens, comparing its features to those of a blood culture (BC). Following evaluation of the performance for sensitivity and specificity of PCR-M, 78 blood samples from 54 patients with suspected bacterial infections were evaluated. Whole-blood samples for PCR-M were collected at the same time as BC, and PCR-M results were compared with BC results. As a result, minimum sensitivity of the kit was 1-100 cfu/ml. The PCR-M test correctly identified specificity for 13 out of 14 strains blinded to the assay analyst. Of 78 blood samples examined, 56 (72%) were negative by both methods, and 22 (28%) were positive by at least one of the two methods. PCR-M detected organisms in 21 cases (27%) compared with 12 cases (15%) in BC. The correlation of positives between PCR-M and BC was 92% (11/12), and both methods identified the same organisms in these 11 cases. With higher positive rate compared with BC, PCR-M could detect and identify potentially significant microorganisms within a few hours by using a small volume of a single whole-blood sample. Early detection of microorganisms has the potential to facilitate early determination of appropriate treatment and antimicrobial selection.
AB - The rapid diagnosis of pathogens and prompt initiation of appropriate antibiotic therapy are critical factors to reduce the morbidity and mortality associated with sepsis. In this study, we evaluated a multiplex polymerase chain reaction (PCR-M) test that detects bacteria and fungi in whole-blood specimens, comparing its features to those of a blood culture (BC). Following evaluation of the performance for sensitivity and specificity of PCR-M, 78 blood samples from 54 patients with suspected bacterial infections were evaluated. Whole-blood samples for PCR-M were collected at the same time as BC, and PCR-M results were compared with BC results. As a result, minimum sensitivity of the kit was 1-100 cfu/ml. The PCR-M test correctly identified specificity for 13 out of 14 strains blinded to the assay analyst. Of 78 blood samples examined, 56 (72%) were negative by both methods, and 22 (28%) were positive by at least one of the two methods. PCR-M detected organisms in 21 cases (27%) compared with 12 cases (15%) in BC. The correlation of positives between PCR-M and BC was 92% (11/12), and both methods identified the same organisms in these 11 cases. With higher positive rate compared with BC, PCR-M could detect and identify potentially significant microorganisms within a few hours by using a small volume of a single whole-blood sample. Early detection of microorganisms has the potential to facilitate early determination of appropriate treatment and antimicrobial selection.
KW - Blood culture
KW - Real-time polymerase chain reaction (RT-PCR)
KW - Sepsis
KW - Systemic inflammatory response syndrome (SIRS)
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U2 - 10.1007/s10156-010-0168-z
DO - 10.1007/s10156-010-0168-z
M3 - Article
C2 - 20976514
AN - SCOPUS:80051586700
SN - 1341-321X
VL - 17
SP - 327
EP - 333
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
IS - 3
ER -