Thermoresponsive bio-affinity interfaces for temperature-modulated selective capture and release of targeted exosomes

Kenichi Nagase, Kaichi Yamazaki, Yutaro Maekawa, Hideko Kanazawa

研究成果: Article査読

3 被引用数 (Scopus)


The existing methods for exosome isolation, such as ultracentrifugation, size exclusion, and affinity separation, suffer from some limitations. Herein, we aimed to develop temperature-modulated exosome-capturing materials using thermoresponsive polymers and peptides with affinity for exosomes. Poly(2-hydroxyethyl methacrylate-co-propargyl acrylate)-b-poly(N-isopropylacrylamide) (P(HEMA-co-PgA)-b-PNIPAAm) was grafted on silica beads via a two-step process of activator regenerated by electron transfer atom transfer radical polymerization. Peptides with affinity for exosomes were conjugated to the propargyl group of the bottom P(HEMA-co-PgA) segment of the copolymer via a click reaction. The prepared copolymer-grafted beads were characterized by elemental analysis, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, gel permeation chromatography, and the turbidity of the polymer solution. Results indicated that the copolymer and peptide were successfully modified on the silica beads. Exosomes from SK-BR-3 ​cells, a human breast cancer cell line, were selectively captured on the prepared beads at 37 ​°C, as the upper PNIPAAm segment shrank and the affinity between the peptide and exosome was enhanced. Upon lowering the temperature to 4 ​°C, the captured exosomes were released from the copolymer brush because of the extension of the PNIPAAm segment that reduced the affinity between peptides and exosomes. These findings demonstrated that the prepared copolymer brush-grafted silica beads can capture and release targeted exosomes via temperature modulation. Taken together, the developed copolymer brush-grafted silica beads would be useful for the separation of exosomes using simple procedures such as temperature modulation.

ジャーナルMaterials Today Bio
出版ステータスPublished - 2023 2月

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体材料
  • 生体医工学
  • 分子生物学
  • 細胞生物学


「Thermoresponsive bio-affinity interfaces for temperature-modulated selective capture and release of targeted exosomes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。