TY - JOUR
T1 - Transcriptome database KK-Periome for periodontal ligament development
T2 - Expression profiles of the extracellular matrix genes
AU - Nishida, Eisaku
AU - Sasaki, Takashi
AU - Ishikawa, Sabine Kazuko
AU - Kosaka, Kazutaka
AU - Aino, Makoto
AU - Noguchi, Toshihide
AU - Teranaka, Toshio
AU - Shimizu, Nobuyoshi
AU - Saito, Masahiro
N1 - Funding Information:
We thank Dr. Toshio Kawase for providing hPDL cells and Dr. Eiro Kubota for help in obtaining human PDL samples. We are also grateful to Drs. Naohito Nozaki, Sachiko Iseki, Takamasa Yokoi and Takanori Tsubakimoto for their helpful advice and discussions during the course of this work. This work was supported by a Grant-in Aid for High-Tech Research Center Project from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) the AGU High-Tech Research Center Project, the 2003 Multidisciplinary Research Project from MEXT, and grants from MEXT.
PY - 2007/12/1
Y1 - 2007/12/1
N2 - Specialized connective tissues such as tendon/ligament develop through a series of events that require temporal and spatial expression of numerous genes in mesenchymal progenitors. However, the genes required for tendon/ligament development have not been identified yet. To solve this problem, we made a cDNA library from periodontal ligament and sequenced 11,520 cDNA clones, as a model for investigating tendon/ligament development. The resulting sequence data was assembled to 617 expressed sequence tag (EST) clusters, and an EST database for human periodontal ligament (PDL) was constructed (designated as the KK-Periome database). In the KK-Periome database, the top 13 EST clusters were related to extracellular matrix (ECM) genes. The temporal and spatial expression patterns of these genes during mouse PDL development were examined by in situ hybridization. Among these genes, F-spondin was expressed specifically in dental follicle (DF) cells during tooth germ development, whereas tenascin-N was strongly expressed in the terminally differentiated PDL. This characteristic expression profile was confirmed by in vivo differentiation assay of human PDL (hPDL) cells in the mouse transplant. Thus, the KK-Periome database was proven to be a useful resource for PDL-derived ESTs (transcriptome), and in fact, initial evidence indicated that F-spondin and tenascin-N might serve as markers for DF and PDL, respectively.
AB - Specialized connective tissues such as tendon/ligament develop through a series of events that require temporal and spatial expression of numerous genes in mesenchymal progenitors. However, the genes required for tendon/ligament development have not been identified yet. To solve this problem, we made a cDNA library from periodontal ligament and sequenced 11,520 cDNA clones, as a model for investigating tendon/ligament development. The resulting sequence data was assembled to 617 expressed sequence tag (EST) clusters, and an EST database for human periodontal ligament (PDL) was constructed (designated as the KK-Periome database). In the KK-Periome database, the top 13 EST clusters were related to extracellular matrix (ECM) genes. The temporal and spatial expression patterns of these genes during mouse PDL development were examined by in situ hybridization. Among these genes, F-spondin was expressed specifically in dental follicle (DF) cells during tooth germ development, whereas tenascin-N was strongly expressed in the terminally differentiated PDL. This characteristic expression profile was confirmed by in vivo differentiation assay of human PDL (hPDL) cells in the mouse transplant. Thus, the KK-Periome database was proven to be a useful resource for PDL-derived ESTs (transcriptome), and in fact, initial evidence indicated that F-spondin and tenascin-N might serve as markers for DF and PDL, respectively.
KW - Connective tissue
KW - Database
KW - EST library
KW - Expression patterning
KW - Extracellular matrix
KW - Periodontal ligament
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U2 - 10.1016/j.gene.2007.09.009
DO - 10.1016/j.gene.2007.09.009
M3 - Article
C2 - 17928168
AN - SCOPUS:35248893485
SN - 0378-1119
VL - 404
SP - 70
EP - 79
JO - Gene
JF - Gene
IS - 1-2
ER -