TY - JOUR
T1 - Transient relaxation of plasmid DNA in Escherichia coli by fluoroquinolones
AU - Matsuo, Miki
AU - Ohtsuka, Yoshihisa
AU - Kataoka, Kazuhiro
AU - Mizushima, Tohru
AU - Sekimizu, Kazuhisa
PY - 1996/9
Y1 - 1996/9
N2 - We examined the influence of fluoroquinolones (norfloxacin, enoxacin, ofloxacin, levofloxacin, and sparfloxacin) on DNA supercoiling of plasmids in Escherichia coli cells by analysis with agarose gel electrophoresis in the presence of chloroquine. All the fluoroquinolones tested immediately induced DNA relaxation. The relaxed DNA was re-supercoiled, and the process was sensitive to chloramphenicol, suggesting that newly synthesized proteins participate in the reaction. The concentrations of fluoroquinolones required for DNA relaxation were much higher than those required for cell killing. The bactericidal effect of fluoroquinolones is apparently related to mechanisms other than DNA relaxation.
AB - We examined the influence of fluoroquinolones (norfloxacin, enoxacin, ofloxacin, levofloxacin, and sparfloxacin) on DNA supercoiling of plasmids in Escherichia coli cells by analysis with agarose gel electrophoresis in the presence of chloroquine. All the fluoroquinolones tested immediately induced DNA relaxation. The relaxed DNA was re-supercoiled, and the process was sensitive to chloramphenicol, suggesting that newly synthesized proteins participate in the reaction. The concentrations of fluoroquinolones required for DNA relaxation were much higher than those required for cell killing. The bactericidal effect of fluoroquinolones is apparently related to mechanisms other than DNA relaxation.
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U2 - 10.1111/j.2042-7158.1996.tb06018.x
DO - 10.1111/j.2042-7158.1996.tb06018.x
M3 - Article
C2 - 8910869
AN - SCOPUS:0029906646
SN - 0022-3573
VL - 48
SP - 985
EP - 987
JO - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
IS - 9
ER -