@article{73623d0317904d1d85f4d9b5915990b6,
title = "Travelling ultrasound promotes vasculogenesis of three-dimensional-monocultured human umbilical vein endothelial cells",
abstract = "To generate three-dimensional tissue in vitro, promoting vasculogenesis in cell aggregates is an important factor. Here, we found that ultrasound promoted vasculogenesis of human umbilical vein endothelial cells (HUVECs). Promotion of HUVEC network formation and lumen formation were observed using our method. In addition to morphological evaluations, protein expression was quantified by western blot assays. As a result, expression of proteins related to vasculogenesis and the response to mechanical stress on cells was enhanced by exposure to ultrasound. Although several previous studies have shown that ultrasound may promote vasculogenesis, the effect of ultrasound was unclear because of unregulated ultrasound, the complex culture environment, or two-dimensional-cultured HUVECs that cannot form a lumen structure. In this study, regulated ultrasound was propagated on three-dimensional-monocultured HUVECs, which clarified the effect of ultrasound on vasculogenesis. We believe this finding may be an innovation in the tissue engineering field.",
keywords = "mechanotransduction, tissue engineering, ultrasound, vasculogenesis",
author = "Chikahiro Imashiro and Tetsuya Azuma and Shun Itai and Taiki Kuribara and Kiichiro Totani and Hiroaki Onoe and Kenjiro Takemura",
note = "Funding Information: This study was supported by JSPS KAKENHI Grant Numbers 16H04259, 17KK0119, 17H07081, 18J12482, and 20J00337 and the Fukuda Foundation for Medical Technology. C. Imashiro and S. Itai are JSPS research fellows. The authors thank to Prof. Tatsuya Shimizu at Tokyo Women's Medical University and Prof. Katsuhisa Sakaguchi at Waseda University for the helpful advice. Funding Information: This study was supported by JSPS KAKENHI Grant Numbers 16H04259, 17KK0119, 17H07081, 18J12482, and 20J00337 and the Fukuda Foundation for Medical Technology. C. Imashiro and S. Itai are JSPS research fellows. The authors thank to Prof. Tatsuya Shimizu at Tokyo Women's Medical University and Prof. Katsuhisa Sakaguchi at Waseda University for the helpful advice. Publisher Copyright: {\textcopyright} 2021 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC",
year = "2021",
month = oct,
doi = "10.1002/bit.27852",
language = "English",
volume = "118",
pages = "3760--3769",
journal = "Biotechnology and bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "10",
}