@inproceedings{b22a43bbc50e4105a1b3b8cf57b84d17,
title = "Tube-Shaped In-Vitro Intestinal Gut Model with 3D Isotropic Medium Supply for Bacterial Symbiosis",
abstract = "This paper describes a tube-shaped in vitro intestinal gut model achieving both isotropic medium supply and stable co-culture with bacteria. Our tube-shaped device consists of a collagen/Matrigel tube as a scaffold for intestinal cells and glass/silicone tubes at both ends for perfusion. We confirmed intestinal cells (Caco-2) can be maintained in the luminal part with nutrient-free buffer flow mimicking bacterial suspensions and demonstrated the co-culture of Escherichia-coli (E. coli) in the Caco-2 cultured tube-shaped device. We believe this device provides ple but stable co-culture model with intestinal cells and bacteria and contributes to medical field research relating intestinal bacteria.",
keywords = "Cell culturing, In vitro 3D tissue, Intestinal bacteria, Micro tube, Tissue engineering",
author = "Mitsuki Kawahara and Shun Itai and Hiroaki Onoe",
note = "Funding Information: This work was partly supported by Moonshot Research & Development Program from Japan Agency for Medical Research and Development (AMED), Japan. Publisher Copyright: {\textcopyright} 2022 IEEE.; 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 ; Conference date: 09-01-2022 Through 13-01-2022",
year = "2022",
doi = "10.1109/MEMS51670.2022.9699523",
language = "English",
series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "321--324",
booktitle = "35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022",
}