@article{6dc924ae0c6942e89d67019bb2929422,
title = "Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells",
abstract = "We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).",
keywords = "Cell Biology, Cell culture, Flow Cytometry/Mass Cytometry, Genomics, Molecular Biology, Single Cell, Stem Cells",
author = "Mitsutoshi Yamada and Tohru Sugawara and Shinju Usami and Rina Nakanishi and Hidenori Akustu",
note = "Funding Information: We thank A. Nakamura and T. Kawasaki for their technical support and laboratory management. M.Y. was supported in part by a KAKENHI grant from MEXT /JSPS ( JP20H03827 Kiban-B-20H03827 ) and a grant from the Takeda Science Foundation . Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = sep,
day = "17",
doi = "10.1016/j.xpro.2021.100811",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "3",
}