Vitreous metabolomics profiling of proliferative diabetic retinopathy

Yohei Tomita, Gael Cagnone, Zhongjie Fu, Bertan Cakir, Yumi Kotoda, Masaki Asakage, Yoshihiro Wakabayashi, Ann Hellström, Jean Sébastien Joyal, Saswata Talukdar, Lois E.H. Smith, Yoshihiko Usui

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Aims/hypothesis: Proliferative diabetic retinopathy (PDR) with retinal neovascularisation (NV) is a leading cause of vision loss. This study identified a set of metabolites that were altered in the vitreous humour of PDR patients compared with non-diabetic control participants. We corroborated changes in vitreous metabolites identified in prior studies and identified novel dysregulated metabolites that may lead to treatment strategies for PDR. Methods: We analysed metabolites in vitreous samples from 43 PDR patients and 21 non-diabetic epiretinal membrane control patients from Japan (age 27–80 years) via ultra-high-performance liquid chromatography-mass spectrometry. We then investigated the association of a novel metabolite (creatine) with retinal NV in mouse oxygen-induced retinopathy (OIR). Creatine or vehicle was administered from postnatal day (P)12 to P16 (during induced NV) via oral gavage. P17 retinas were quantified for NV and vaso-obliteration. Results: We identified 158 metabolites in vitreous samples that were altered in PDR patients vs control participants. We corroborated increases in pyruvate, lactate, proline and allantoin in PDR, which were identified in prior studies. We also found changes in metabolites not previously identified, including creatine. In human vitreous humour, creatine levels were decreased in PDR patients compared with epiretinal membrane control participants (false-discovery rate <0.001). We validated that lower creatine levels were associated with vascular proliferation in mouse retina in the OIR model (p = 0.027) using retinal metabolomics. Oral creatine supplementation reduced NV compared with vehicle (P12 to P16) in OIR (p = 0.0024). Conclusions/interpretation: These results suggest that metabolites from vitreous humour may reflect changes in metabolism that can be used to find pathways influencing retinopathy. Creatine supplementation could be useful to suppress NV in PDR. [Figure not available: see fulltext.]

本文言語English
ページ(範囲)70-82
ページ数13
ジャーナルDiabetologia
64
1
DOI
出版ステータスPublished - 2021 1月

ASJC Scopus subject areas

  • 内科学
  • 内分泌学、糖尿病および代謝内科学

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